Juventus on Monday were given a 10-point deduction in Serie A after a revision of their initial 15-point penalty inflicted on the club over illicit transfer activity.
The new sanction, announced by the Italian Football Federation's (FIGC) appeals court, severely dents Juventus' hopes of qualifying for next season's Champions League, pushing them out of the top four with two matches remaining this season.
Juventus have competed in Europe's elite competition in each of the past 11 seasons dating back to 2012. In April, Italy's highest sporting court, the Sports Guarantee Board, revoked Juve's 15-point penalty inflicted by the FIGC's appeals court and sent the case for a new judgement.
It asked the appeals court to revise punishments handed down to the club and some key figures, including former vice-chairman Pavel Nedved who was acquitted on Monday.