Australia saved their best for the last as Indian middle-order wilted under pressure exerted by part-time off-spin of Glenn Maxwell, losing the third ODI by 66 runs on Wednesday.
India however won the series 2-1. India were cruising along at 171 for 2 chasing a stiff target of 353 on a placid track but the middle-order crumbled in chase with Maxwell (4/40) getting crucial breakthroughs to restrict the hosts to 286 in 49.4 overs.
Skipper Rohit Sharma (81) and Kohli struck fifties to provide the middle-order with a perfect platform in the big chase, but the rest floundered to hand Australia the advantage.
Earlier, Mitchell Marsh (96) headlined Australia's best batting show with David Warner, Steve Smith and Marnus Labuschagne helping themselves to attacking half-centuries during a commanding performance to break a five-ODI losing streak.