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Elliot Daley, Dan Cole, Max Malins and Ben Curry were included in England coach Steve Borthwick's Six Nations squad on Monday having been dropped by Eddie Jones but there was no place for wingers Jonny May and Jack Nowell or number eight Billy Vunipola.
Martin Gleeson has left his role as England attack coach, the RFU said on Friday.
Flanker Tom Curry has been ruled out of England's first two Six Nations games after suffering a hamstring tear in Sale Sharks' Premiership win over Harlequins on Sunday.
Steve Borthwick has been dealt another pre-Six Nations injury blow with Sale flanker Tom Curry set to miss England’s first two games.
Owen Farrell will be available for the start of England's Six Nations against Scotland on 4 February after receiving a four-match ban for a dangerous tackle.
Exeter hooker Luke Cowan-Dickie will miss at least the start of England's Guinness Six Nations campaign after suffering an ankle injury.
England hooker Luke Cowan-Dickie is likely to require surgery on an ankle injury and is set to miss the start of the Six Nations.
Owen Farrell is in danger of missing the start of England’s Six Nations campaign after being cited for a dangerous tackle in Saracens’ victory over Gloucester on Friday.